Lee Clark's men needed Brighton to avoid defeat at the New York Stadium and beat Reading on Tuesday to keep their slim hopes of survival alive.
It was not to be, though, as Matt Derbyshire fired Steve Evans' men to victory in what is a precious three points in Rotherham's own fight against an immediate return to League One - the South Yorkshire club now seven points clear of the relegation zone.
Blackpool's drop into League One has been on the cards for some time, with the club having endured a miserable season in which they have won just four matches and failed to win away from Bloomfield Road this season - their last win having coming against Brighton in January.
'People will remember us because we got to No.1 in the world rankings and achieved great things - but our biggest regret is that we didn’t win anything': Ex-Everton star Kevin Mirallas opens up on 'honour' of being part of nation's golden generation
‘I’m sure we’d have finished in the top four or five if he hadn’t joined Chelsea in January. Him leaving damaged the team, but we couldn’t stand in his way’: Alan Curbishley reflects on failed Champions League dream after selling key player
