Phillip Cocu's PSV bounced back from defeat to AZ Alkmaar in their last Eredivisie match with a last-gasp 2-1 win over struggling RKC Waalwijk, a result that moves them level on points with Twente.
PSV were made to work hard for their triumph, with Jurgen Locadia firing home their winner in the 88th minute just moments after Sander Duits had been dismissed for a second bookable offence.
Ola Toivonen had put PSV ahead only for Robert Braber to pull Waalwijk level on the stroke of half-time.
Ajax sit one point behind the top two after a comfortable 3-0 home triumph over FC Utrecht.
Thulani Serero opened the scoring shortly before the break and Davy Klaassen doubled Ajax's lead in the 59th minute.
Danny Hoesen then sealed the result with a third goal in the dying stages.
Early strikes from Stefan de Vrij and Graziano Pelle proved enough for Feyenoord as they moved up to fourth with a 2-1 win at Vitesse.
De Vrij and Pelle found the net in the space of three first-half minutes to give their side a handy cushion.
Vitesse set up a tense finish through Mike Havenaar's 83rd-minute strike but were unable to find an equaliser.
In the day's other fixture, Groningen beat AZ Alkmaar 2-1 to move above their opponents and into eighth.
Johann Gudmundsson wasted little time in cancelling out a 52nd-minute own goal from AZ team-mate Nick Viergever.
Yet Groningen were not to be denied and claimed three points courtesy of a third goal this season from 17-year-old forward Richairo Zivkovic.
‘The pure simplicity of the way Slot has managed the squad is probably the biggest thing I could say about him. It’s not broken, so let’s get on with it’: Liverpool legend full of admiration for Jurgen Klopp's successor at Anfield
‘I trained at Spurs and thought they’d give me a chance. But I received a letter thanking me but saying they didn’t think I was good enough – I was gutted’: How Tottenham missed out on signing Wales legend John Toshack
