Chelsea were responsible for the biggest outlay, with significant activity following the return of manager Jose Mourinho taking their spend to a whopping £13,721,721.
Manchester City, Tottenham and Liverpool were the other major spenders, while Crystal Palace, who paid £869,531, are the only top-flight team to have spent less than £1m over the 12-month period.
The total spend of £96.67m represents a new high and an increase of almost £20m on last year's corresponding figure.
Southampton, the surprise package of the season to date, were among the lowest spenders, paying just £2,184,412 to agents.
Premier League leaders Arsenal and reigning champions Manchester United posted relatively modest outgoings of £5,485,961 and £4,317,690 having made limited additions in the recent transfer window.
Total money spent on agents by Premier League clubs between October 1 2012 and September 30 2013:
Arsenal: £5,485,961
Aston Villa: £3,358,628
Cardiff City: £2,225,582
Chelsea: £13,721,721
Crystal Palace: £869,531
Everton: £3,225,159
Fulham: £3,790,115
Hull City: £1,825,718
Liverpool: £9,400,973
Manchester City: £11,179,817
Manchester United: £4,317,690
Newcastle United: £7,294,018
Norwich City: £2,308,987
Southampton: £2,184,412
Stoke City: £3,191,808
Sunderland: £4,640,227
Swansea City: £1,484,878
Tottenham: £9,787,676
West Brom: £2,211,054
West Ham: £4,169,134
TOTAL: £96,673,089
