The Portugal international was sent off in the 1-1 draw with Athletic Bilbao earlier this month, having appeared to strike Carlos Gurpegi in the face following an altercation between the pair.
Real appealed the Spanish Football Federation's three-match ban and, when this was rejected, took their case to the Ministry for Education, Culture and Sport - who also dismissed the case.
Carlo Ancelotti expressed his frustration at the lengthy appeal process on Friday, and claimed he had included Ronaldo in his match-day squad, hinting at a confidence the ban would be overturned.
Having already missed league clashes with Villarreal and Getafe, Ronaldo will now serve the final game of the original ban when Madrid take on Elche.
‘He could have been another Thierry Henry if he’d stayed at Arsenal. It was up to him what he wanted because he was 6ft 1in, quick and unbelievable’: Ray Parlour claims Champions League winner could have enjoyed even greater success with Gunners
‘I don’t think we’ll see Marcus Rashford at Manchester United again – when you’re not seeing eye to eye with a manager, it can hinder your training, but a loan can work, it did for me’ Ex-Premier League star on parallels with forward’s Aston Villa switch
